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Tender Details
- Country: United Kingdom
- Summary: Rbgkew1309 Long-Read Ash Genome Sequencing 2024
- GBT Ref No: 101170768
- Deadline: 13 Jun 2024
- Competition: ICB
- Financier: Self Financed
- Purchaser Ownership: Public
- Tender Value: 167000
- Notice Type: Tender
- Document Ref. No.: BIP861438760
- Purchaser's Detail :
- Description :
- Title : RBGKEW1309 Long-Read Ash Genome Sequencing 2024
Location Of Contract : London
Industry : Research services - 73110000
Value Of Contract : £167,000
Description : Royal Botanic Gardens, Kew wishes to appoint a supplier to individually sequence the whole genomes of up to 300 European ash trees (Fraxinus excelsior) at a minimum of 15x whole genome coverage via a long-read technology. Fraxinus excelsior is a diploid and has a 1C genome size of 880 Mbp. In addition to the 300 individual ash trees, we wish to sequence a minimum of six technical replicates. Therefore, the total number of samples we wish to sequence is 306. RBG Kew intends to supply total genomic DNA for each sample with the following metrics: minimum of 10ug DNA; minimum concentration of 30ng/ml (as measured with a Quantus flurometer dsDNA assay); at least 50% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1.8; minimum OD260/230 of 1.8; minimum DIN of 7.
Published Date : 13 May 2024
Closing Date : 13 June 2024 - Documents :
Purchaser : ROYAL BOTANIC GARDENS, KEW
The Herbarium, Kew Green Richmond, London TW9 3AE UK
Email :procurement@kew.org
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