Tender Details

Country: United Kingdom
Summary: Gb-London: Rbgkew1276 - Whole Genome Sequencing for Cacao Dna from Herbarium Specimens
GBT Ref No: 95374038
Deadline: 02 Feb 2024
Financier: Self Financed
Purchaser Ownership: Government
Tender Value: GBP 50000
Notice Type: Tender
Document Ref. No.: BIP836041993
Purchaser's Detail:
Login to see full purchaser details. Login to see full purchaser details. Login to see full purchaser details. Login to see full purchaser details. Login to see full purchaser details. Login to see full purchaser details. Login to see full purchaser details. Login to see full purchaser details. Login to see full purchaser details.
Login to see details
Description:
Title : GB-London: RBGKEW1276 - Whole Genome Sequencing for cacao DNA from herbarium specimens
Location Of Contract : London
Industry : Research laboratory services - 73111000
Value Of Contract : £25,000 to £50,000
Description : The Royal Botanic Gardens, Kew (RBG Kew) intends to appoint a supplier for a high-throughput sequencing service from ancient-DNA extractions sourced from historical herbarium collections of cacao specimens (Theobroma cacao, Malvaceae) and wild relative species (Theobroma/Herrania spp.). Utilizing a CTAB-extraction method, the extracted samples will be sent in batches. The supplier must conduct pre-quality control (QC) tests (including RNase treatment and DNA purification, if necessary) on these total-genome DNA extractions, ensuring acceptable yields and concentrations. Tasks also include library preparation using the Nextera XT Library Kit and performing paired-end whole genomic sequencing (WGS) using the Illumina NovaSeq 6000 (or an equally powerful sequencing platform) to seek obtaining ideally ~12 Gb per sample, depending on the conditions of the sample. This process aims to cover 120-140 samples, targeting a coverage of 15-30x each, given the known genome size of Theobroma species at ~450-490 Mbp. The supplier should be acquainted with high-throughput sequencing from aged herbarium samples, due to the historical nature of these collections (preserved over 50-100 years ago)...
Documents:

If you are registered member, kindly login to view full details of this tender notice:

Gb-London: Rbgkew1276 - Whole Genome Sequencing for Cacao Dna from Herbarium Specimens - Uk Tender

The BIP SOLUTIONS LIMITED, a Government sector organization in United Kingdom, has announced a new tender for Gb-London: Rbgkew1276 - Whole Genome Sequencing for Cacao Dna from Herbarium Specimens. This tender is published on UkTender under GBT Ref No: 95374038 and is categorized as a Tender. Interested and eligible suppliers are invited to participate by reviewing the tender documents and submitting their bids before the deadline on 2024-02-02.

The estimated tender value is GBP 50000, and full details, including technical specifications and submission requirements, are provided in the official tender documents. Ensure all submissions meet the criteria outlined to be considered for evaluation.