Tender Details

Country: United Kingdom
Summary: Cefas24-126 Rfq for Genotype-By-Sequencing Approach of Colossal Squid
GBT Ref No: 107134562
Deadline: 30 Sep 2024
Financier: Self Financed
Purchaser Ownership: Government
Tender Value: GBP 30000
Notice Type: Tender
Document Ref. No.: CF-0364600D8d000003VQwdEAG
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Description:
Cefas is currently undertaking a population genetics assessment on colossal squid. Cefas is seeking a supplier to sequence rare samples using a genotype-by-sequencing (GBS) approach. Tissue samples (mainly tentacles) have been collected from various regions across the globe. The samples themselves are in two types of preservative, depending on the institutes which collected the samples; ethanol and formaldehyde.

Cefas is aware that there is a multitude of genotype-by-sequencing approaches, some using propriety technology and some not. We are seeking applications which offer a GBS approach that maximises SNP identification and coverage to allow Cefas explore population structure. Additionally, suppliers must have a comprehensive DNA extraction step to compensate for varying levels of sample preservation.

Due to the scarcity of these animals, we have 33 samples but we expect samples to be run in duplicate at an average coverage of x30 depth for each duplicated sample.

Requirement:
To aid with the above project, Cefas require DNA extraction of squid tissues in ethanol (31) and formaldehyde (2). Assessment of DNA quantity and quality (see 'Deliverables' sections) will need to be completed and discussed with Cefas before genotype-by-sequencing of squid samples begins. Cefas are seeking the highest sequencing coverage possible to compensate for minimum samples sizes. Therefore, suppliers are asked to provide a cost per individual sample (n=66, in total).
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Documents:

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Cefas24-126 Rfq for Genotype-By-Sequencing Approach of Colossal Squid - Uk Tender

The CEFAS, a Government sector organization in United Kingdom, has announced a new tender for Cefas24-126 Rfq for Genotype-By-Sequencing Approach of Colossal Squid. This tender is published on UkTender under GBT Ref No: 107134562 and is categorized as a Tender. Interested and eligible suppliers are invited to participate by reviewing the tender documents and submitting their bids before the deadline on 2024-09-30.

The estimated tender value is GBP 30000, and full details, including technical specifications and submission requirements, are provided in the official tender documents. Ensure all submissions meet the criteria outlined to be considered for evaluation.